Composite

Part:BBa_K4103022:Design

Designed by: Almiro Pires da Silva Neto   Group: iGEM21_Open_Science_Global   (2021-10-20)


Secretion Tag library 1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1878
    Illegal PstI site found at 2242
    Illegal PstI site found at 3708
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1878
    Illegal PstI site found at 2242
    Illegal PstI site found at 3708
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1878
    Illegal PstI site found at 2242
    Illegal PstI site found at 3708
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1878
    Illegal PstI site found at 2242
    Illegal PstI site found at 3708
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1533
    Illegal BsaI site found at 1730
    Illegal BsaI site found at 1972
    Illegal BsaI site found at 2172
    Illegal BsaI site found at 2375
    Illegal BsaI site found at 2596
    Illegal BsaI site found at 2793
    Illegal BsaI.rc site found at 1656
    Illegal BsaI.rc site found at 1898
    Illegal BsaI.rc site found at 2098
    Illegal BsaI.rc site found at 2301
    Illegal BsaI.rc site found at 2522
    Illegal BsaI.rc site found at 2719
    Illegal BsaI.rc site found at 2925


Design Notes

Sequence has been modified, with a couple of sec tag CDSs recoded, and several random spacers replaced with other random sequences, in order to remove potential repeats and hairpins in the sequence that were flagged by Twist.


Source

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References